Ron Lab


A note on using SV40 transformed mutant MEFs

30 October 2013

We are unable to meet the demand for I˚ MEFs. Therefore the cells we send out and those deposited at ATCC are SV40 large T-antigen transformed.

Please note that immortalized cells have undergone lengthy adaptation to tissue culture and some phenotypic features, such as the hypersensitivity to ER stress may have been attenuated. As a consequence wildtype transformed MEFS are a poor control for the analysis of complex phenotypes in the mutant cells. This is true even if the parental stock of wildtype and mutant MEFS were highly comparable (i.e. even if the two cell populations were isogenic at their origin).

The only thing that can be stated with confidence in regards to these knock-out cells is that they lack the gene in question. Thus they may be useful loss-of-function model for proximal signaling events that cannot be easily compensated for by adaptation in function and activity of other genes (trans-suppresion).