Ron Lab


 

ATF4 Glycerol Stocks

These plasmids were used in Phoebe Lu's paper: Lu PD, Harding HP and Ron D. 2004. Translation re-initiation at alternative open reading frames regulates gene expression in an integrated stress response. J Cell Biol. 167:27-33.

Important Note: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin).


JCB Name Ron Lab Name E. coli
Strain
Glycerol Stock Date Glycerol Stock Location
ATF4 4: GFP pEGFP-N1 Stbl2 6/16/01 Box 17, #89
ATF4 5: 5’ATF4.GFP 5’ATF4.MscI.pEGFP-N1 DH5a 10/27/03 Box 20, #66
ATF4 6: mCD36.GFP mCD36.2.pEGFP-N2 DH5a 2/25/04 Box 20, #73
ATF4 7: 5’3’ATF4.GFP 5’3’ATF4.pEGFP-N1C2.V1 Stbl2 12/5/03 Box 20, #67
ATF4 8: (SL-)5’ATF4.GFP (SL-)5’ATF4.pEGFP-N1 DH5a 4/12/04 Box 21, #32
ATF4 9: (SL+)5’ATF4.GFP (SL+)5’ATF4.pEGFP-N1 DH5a 4/12/04 Box 21, #33
ATF4 10: 5’ATF4.uORF1.GFP 5’ATF4.uORF1.pEGFP-N2 Stbl2 1/30/04 Box 20, #71
ATF4 11: 5’ATF4.uORF2.GFP 5’ATF4.uORF2.pEGFP-N2 DH5a 2/25/04 Box 20, #72
ATF4 12: 5’ATF4.uORF1AUA.GFP 5’ATF4.muORF1ATA.pEGFP-N1 DH5a 2/25/04 Box 20, #68
ATF4 13: 5’ATF4.uORF2AUA.GFP 5’ATF4.muORF2ATA.pEGFP-N1 DH5a 2/25/04 Box 20, #69
ATF4 14: 5’ATF4.uORF1&2AUA.GFP 5’ATF4.muORFs1&2ATA.pEGFP-N1 DH5a 3/1/04 Box 20, #70
ATF4 15: 5’ATF4.uORF1stopm1.GFP 5’ATF4.muORF1stop1.pEGFP-N1 DH5a 4/20/04 Box 21, #34
ATF4 17: 5’ATF4.uORF1stopm1&2.GFP 5’ATF4.muORF1stop1&2.pEGFP-N1 DH5a 4/20/04 Box 21, #35
ATF4 18: RLuc.5’ATF4.GFP RLucATF4-EGFP.pcDNA3 Stbl2 12/23/03 Box 20, #64
ATF4 19: (SL)RLuc.5’ATF4.GFP (SL)RLucATF4-EGFP.pcDNA3 Stbl2 12/23/03 Box 20, #65