Ron Lab


 

9E10-tagged wildtype mouse PERK in the pro-retroviral vector pBABEpuro

Mammalian expression plasmid encoding wildtype mouse PERK fused to 9E10 epitope at C-term.

Plasmid sequence in EMBL format:

FT CDS 1573..4944
FT /codon_start=1
FT CDS complement(8498..7638)
FT /gene="AmpR"/product="beta lactamase"
FT LTR 1..460
FT /gene="5'
FT LTR"/note="The left LTR serves as the promoter of
FT the subcloned
FT LTR 5957..6582
FT /gene="3'
FT LTR"/product="3' LTR"
FT misc_binding 3424..3426
FT /note="ATP binding"
FT misc_feature 1369..1381
FT /note="LINKER"
FT misc_feature 4911..4945
FT /note="9E10-Xho added"
FT misc_feature 6879..6898
FT /gene="Origin"/product="origin of replcation"
FT primer 3406..3437
FT /note="PERK2.(K/A).1"
FT primer 4893..4909
FT /note="PERK2.9E10.1"
FT primer 5001..5024
FT /note="pBABE.2AS"
FT primer 1311..1334
FT /note="BABEP.1S"
FT promoter 4953..5295
FT /gene="SV 40 early
FT promoter"/product="SV 40 early promoter"
FT CDS 5300..5899
FT /gene="Puro-R"/product="puromycin N-acetyltransfe
FT rase"
FT /codon_start=1
FT misc_feature 920..1309
FT /gene="gag"/note="the start codon of the gag gene
FT has been mutated sothat it does not get
FT expressed. Its presence, however,increases the
FT level of retroviral production"
FT primer 3175..3202
FT /note="PERK2.Sal.1S"
FT sig_peptide 3133..3180
FT /note="hydrophobic patch"
XX
SQ Sequence 8645 BP; 1999 A; 2394 C; 2327 G; 1924 T; 1 Other;
CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG GCAAGCTGGC CAGCAACTTA TCTGTGTCTG TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCTGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTTGGGGGC CGTTTTTGTG GCCCGACCTG AGGAAGGGAG TCGATGTGGA ATCCGACCCC GTCAGGATAT GTGGTTCTGG TAGGAGACGA GAACCTAAAA CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGTTTGGA ACCGAAGCCG CGCGTCTTGT CTGCTGCAGC GCTGCAGCAT CGTTCTGTGT TGTCTCTGTC TGACTGTGTT TCTGTATTTG TCTGAAAATT AGGGCCAGAC TGTTACCACT CCCTTAAGTT TGACCTTAGG TCACTGGAAA GATGTCGAGC GGATCGCTCA CAACCAGTCG GTAGATGTCA AGAAGAGACG TTGGGTTACC TTCTGCTCTG CAGAATGGCC AACCTTTAAC GTCGGATGGC CGCGAGACGG CACCTTTAAC CGAGACCTCA TCACCCAGGT TAAGATCAAG GTCTTTTCAC CTGGCCCGCA TGGACACCCA GACCAGGTCC CCTACATCGT GACCTGGGAA GCCTTGGCTT TTGACCCCCC TCCCTGGGTC AAGCCCTTTG TACACCCTAA GCCTCCGCCT CCTCTTCCTC CATCCGCCCC GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT CACTCCTTCT CTAGGCGCCG GCCGGATCCC CAGTGTGGTG GTACGTAGAA TTCGGCACGA GCGATGTCTG CACAAGGCTG TCACTCAGGT GGCAGTGGCT GACACGTGGC CGGGCAGCTC TGCTGCTGCG GCGCGAAGTC GAGAGGCGGC GGGGTCCGTG GCGCGCGCTC GCATTGCTCC GAGGCTCCGA GCGGCGATAC GGGCGGGCGC CGACGGCAGG GTCTCCATGC CCGCGCGTGG GGCGGGCCGC TGATGGAGCG CGCCACCCGG CCCGGGCCGC GCGCGCTGCT GCTGCTTCTG TTCCTGCTGC TGGGCTGCGC GGCGGGGATC TCTGCGGTCG CGCCCGCCCG CAGTTTGCTT GCTCCCGCGT CGGAGACAGT GTTTGGCTTA GGGGCAGCGG CCGCCCCGAC TTCGGCCGCG CGGGTGCCTG CGGTGGCAAC GGCCGAAGTG ACCGTGGAGG ACGCCGAGGC ATTGCCGGCT GCCGCTGGCG AACCGGAGTC ACGCGCGACG GAGCCCGATG ACGACGTGGA ACTGCGGCCT CGCGGCAGGT CCTTGGTAAT CATCAGCACT TTAGATGGAC GAATCGCTGC ACTGGATGCC GAGAATGATG GGAAAAAGCA GTGGGATTTG GACGTGGGGT CTGGTTCCTT GGTTTCATCT AGCCTCAGCA AGCCAGAGGT GTTTGGGAAC AAGATGATCA TCCCCTCCCT GGATGGAGAC CTCTTCCAGT GGGACCGGGA CCGAGAGAGC ATGGAGGCCG TCCCCTTCAC GGTGGAGTCC CTGCTCGAAT CTTCCTACAA GTTTGGAGAT GATGTTGTTC TGGTTGGAGG GAAATCTCTG ATTACATACG GACTCAGTGC TTACAGTGGA AAGCTGAGGT ATATCTGTTC TGCCTTGGGA TGTCGCCGAT GGGATAGTGA TGAAATGGAA GAAGAGGAAG ACATCTTGCT TCTGCAGCGT ACGCAGAAGA CTGTGCGAGC TGTCGGGCCT CGAAGCGGCA GTGAGAAGTG GAATTTCAGT GTTGGCCACT TTGAACTTCG GTATATTCCA GACATGGAAA CTAGAGCCGG ATTCATTGAA AGCACCTTTA AACCGGGTGG AAACAAAGAA GACTCTAAAA TTATTTCAGA TGTGGAAGAA CAAGAAGCCA CCATGCTGGA CACAGTGATA AAAGTTTCCG TTGCTGATTG GAAGGTCATG GCGTTTAGTA GGAAGGGAGG CCGCCTGGAA TGGGAGTACC AGTTTTGTAC TCCCATCGCG TCCGCCTGGC TGGTGAGGGA TGGCAAGGTC ATCCCCATCA GCCTGTTTGA TGATACAAGT TACACAGCCA GCGAAGAAGC CTTGGGAGAC GAAGAAGACA TTGTAGAGGC TGCTCGGGGA GCCACAGAGA ACAGCGTGTA CTTAGGGATG TACAGAGGCC AGCTGTACCT GCAGTCGTCC GTCAGGGTCT CAGAAAAGTT CCCTACAAGC CCAAAGGCCT TGGAGTCTGT AAATGGCGAA AATGCAATTA TTCCTCTGCC GACGATCAAA TGGAAGCCCT TAATCCATTC TCCTTCTAGG ACTCCTGTCT TGGTTGGGTC TGATGAATTT GACAAATGTC TAAGTAATGA TAAGTATTCC CACGAAGAAT ACAGTAATGG TGCACTTTCA ATCCTCCAGT ATCCATACGA TAACGGTTAC TATCTGCCAT ACTACAAGAG AGAAAGGAAT AAGCGGAGCA CGCAGATCAC AGTCAGGTTC CTGGACAGCC CCCACTACAG CAAGAACATC CGCAAGAAGG ACCCTATCCT CCTGCTGCAC TGGTGGAAGG AGATATTCGG GACGATCCTG CTTTGCATCG TAGCCACGAC CTTCATCGTG CGCAGGCTTT TCCATCCTCA GCCCCACAGG CAGCGGAAGG AGTCTGAAAC TCAGTGCCAG ACTGAAAGTA AATACGACTC CGTGAGTGCC GATGTCAGTG ACAACAGCTG GAATGACATG AAGTACTCAG GATACGTATC CCGATATCTA ACAGATTTTG AGCCAATTCA GTGCATGGGT CGTGGTGGCT TTGGCGTTGT CTTTGAAGCT AAAAACAAAG TAGATGACTG CAATTACGCT ATCAAGAGGA TCCGGCTCCC CAACAGGGAG TTGGCACGGG AGAAGGTAAT GCGGGAAGTT AAAGCCTTGG CTAAGCTGGA ACACCCAGGC ATTGTGAGGT ATTTCAACGC CTGGCTGGAA ACCCCACCAG AGAAGTGGCA AGAAGAGATG GATGAGATCT GGCTCAAAGA CGAAAGCACA GACTGGCCGC TCAGCTCCCC TAGCCCGATG GATGCCCCAT CTGTTAAGAT CCGAAGGATG GATCCYTTCT CTACAAAAGA GCAGATCGAA GTCATAGCTC CTTCTCCTGA AAGAAGTCGG TCTTTCTCGG TGGGCATTTC CTGTGGCCAG ACAAGCTCAT CGGAGAGCCA GTTCTCTCCC CTGGAGTTCT CAGGGACAGA CTGCGGAGAC AACAGTGACT CAGCGGACGC AGCCTACAAC CTCCAGGACA GTTGCCTGAC GGACTGCGAG GACGTGGAAG ATGGCACCGT GGACGGCAAT GACGAGGGAC ACTCCTTTGA ACTTTGTCCG TCCGAAGCTT CTCCCTATAC CCGGTCTAGG GAAGGAACGT CCTCCTCCAT AGTGTTTGAG GACTCTGGCT GCGGCAACGC GTCCAGTAAG GAGGAGCCCA GAGGGAACCG GCTGCATGAT GGCAACCATT ATGTTAATAA GCTAACTGAT CTCAAGTGCT CCAGCAGCAG GTCTTCTTCA GAAGCCACCA CCTTGTCTAC CTCCCCTACC AGGCCAACCA CTCTAAGCTT GGATTTCACC AAGAACACTG TGGGCCAGCT CCAGCCCAGC TCCCCCAAGG TGTATCTGTA CATTCAGATG CAGCTGTGCA GGAAGGAGAA CCTCAAAGAC TGGATGAACC GGCGCTGCAG CTTGGAGGAC CGGGAGCACG GCGTGTGCCT GCACATCTTC CTGCAGATCG CAGAGGCAGT GGAGTTCCTG CACAGCAAGG GACTCATGCA CAGGGACCTC AAGCCTTCCA ACATATTCTT CACAATGGAT GATGTGGTCA AGGTTGGGGA CTTTGGACTG GTGACTGCTA TGGACCAAGA TGAAGAAGAG CAGACTGTTC TGACTCCAAT GCCAGCCTAT GCTACGCACA CGGGACAAGT AGGGACCAAG CTATACATGA GCCCAGAGCA GATTCATGGA AACAACTACT CCCATAAAGT GGACATCTTC TCTTTAGGCT TGATTCTGTT TGAACTCCTC TACCCATTCA GCACCCAGAT GGAACGAGTC CGGATTTTAA CTGATGTCAG AAATCTCAAG TTTCCTCTAC TGTTCACTCA GAAATATCCC CAAGAGCATA TGATGGTTCA AGACATGCTC TCTCCATCCC CCACGGAGCG GCCTGAAGCC ACAGACATCA TTGAAAATGC CATATTTGAG AACTTGGAGT TTCCCGGGAA AACGGTTCTG AGACAGCGGT CCCGCTCCAT GAGTTCATCT GGAACAAAAC ATTCCAGACA GCCCAGCTGC TCGTACACGC CACTGCCTGG CGAGCAGAAG CTGATCTCTG AGGAGGATCT GTGACTCGAC CCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC ACGACCCCAT GCATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TCAGGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAC ATGCAGCATG TATCAAAATT AATTTGGTTT TTTTTCTTAA GTATTTACAT TAAATGGCCA TAGTTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTGC GGCCGCAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGC AGCCTGAATA TGGGC

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