Ron Lab


 

Mouse PERK (537-1114), wild-type, 9E10-tagged at C-terminus, fused to GST, bacterial expression vector, pGEX4T-1 (Pharmacia)

PERKKD-pGEX4T-1 (6730nt, predicted). Insert between Sal1 and Xho1:

AGCTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCGGA ATTCCCGGGT CGACGGCTTT TCCATCCTCA GCCCCACAGG CAGCGGAAGG AGTCTGAAAC TCAGTGCCAG ACTGAAAGTA AATACGACTC CGTGAGTGCC GATGTCAGTG ACAACAGCTG GAATGACATG AAGTACTCAG GATACGTATC CCGATATCTA ACAGATTTTG AGCCAATTCA GTGCATGGGT CGTGGTGGCT TTGGCGTTGT CTTTGAAGCT AAAAACAAAG TAGATGACTG CAATTACGCT ATCAAGAGGA TCCGGCTCCC CAACAGGGAG TTGGCACGGG AGAAGGTAAT GCGGGAAGTT AAAGCCTTGG CTAAGCTGGA ACACCCAGGC ATTGTGAGGT ATTTCAACGC CTGGCTGGAA ACCCCACCAG AGAAGTGGCA AGAAGAGATG GATGAGATCT GGCTCAAAGA CGAAAGCACA GACTGGCCGC TCAGCTCCCC TAGCCCGATG GATGCCCCAT CTGTTAAGAT CCGAAGGATG GATCCYTTCT CTACAAAAGA GCAGATCGAA GTCATAGCTC CTTCTCCTGA AAGAAGTCGG TCTTTCTCGG TGGGCATTTC CTGTGGCCAG ACAAGCTCAT CGGAGAGCCA GTTCTCTCCC CTGGAGTTCT CAGGGACAGA CTGCGGAGAC AACAGTGACT CAGCGGACGC AGCCTACAAC CTCCAGGACA GTTGCCTGAC GGACTGCGAG GACGTGGAAG ATGGCACCGT GGACGGCAAT GACGAGGGAC ACTCCTTTGA ACTTTGTCCG TCCGAAGCTT CTCCCTATAC CCGGTCTAGG GAAGGAACGT CCTCCTCCAT AGTGTTTGAG GACTCTGGCT GCGGCAACGC GTCCAGTAAG GAGGAGCCCA GAGGGAACCG GCTGCATGAT GGCAACCATT ATGTTAATAA GCTAACTGAT CTCAAGTGCT CCAGCAGCAG GTCTTCTTCA GAAGCCACCA CCTTGTCTAC CTCCCCTACC AGGCCAACCA CTCTAAGCTT GGATTTCACC AAGAACACTG TGGGCCAGCT CCAGCCCAGC TCCCCCAAGG TGTATCTGTA CATTCAGATG CAGCTGTGCA GGAAGGAGAA CCTCAAAGAC TGGATGAACC GGCGCTGCAG CTTGGAGGAC CGGGAGCACG GCGTGTGCCT GCACATCTTC CTGCAGATCG CAGAGGCAGT GGAGTTCCTG CACAGCAAGG GACTCATGCA CAGGGACCTC AAGCCTTCCA ACATATTCTT CACAATGGAT GATGTGGTCA AGGTTGGGGA CTTTGGACTG GTGACTGCTA TGGACCAAGA TGAAGAAGAG CAGACTGTTC TGACTCCAAT GCCAGCCTAT GCTACGCACA CGGGACAAGT AGGGACCAAG CTATACATGA GCCCAGAGCA GATTCATGGA AACAACTACT CCCATAAAGT GGACATCTTC TCTTTAGGCT TGATTCTGTT TGAACTCCTC TACCCATTCA GCACCCAGAT GGAACGAGTC CGGATTTTAA CTGATGTCAG AAATCTCAAG TTTCCTCTAC TGTTCACTCA GAAATATCCC CAAGAGCATA TGATGGTTCA AGACATGCTC TCTCCATCCC CCACGGAGCG GCCTGAAGCC ACAGACATCA TTGAAAATGC CATATTTGAG AACTTGGAGT TTCCCGGGAA AACGGTTCTG AGACAGCGGT CCCGCTCCAT GAGTTCATCT GGAACAAAAC ATTCCAGACA GCCCAGCTGC TCGTACACGC CACTGCCTGG CGAGCAGAAG CTGATCTCTG AGGAGGATCT GTGACTCGAG CGGCCGCATC GTGACTGACT GACGATCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAAATTCC GACACCATCG AATGGTGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG ACAGCTCATG TTATATCCCG CCGTCAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGGA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTAACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG CGTTGGAATT