Ron Lab


 

Mouse IRE1β, K536A, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, mammalian expression vector, pCDNA3

The K->A mutation introduces a second MSc1 site into the plasmid at position 2578.

IRE1β(K536A).9E10.pCDNA3 (8219nt, predicted). Insert between EcoR1 and Xho1:

GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CCACTAGTAA CGGCCGCCAG TGTGCTGGAA TTCGGCACGA GGAGACTTTG TGGTTGCCCA GACTATGGCG AGACCAGTCC AGAGATTTCA ACTGTGGTCT CCTCTCGGGT TCCTGCTGCA GCTTGTGACG TTGCTGGGGA AACTCGGTCC ACAGGTCCAG TCTGTCAGGC CAGAGAGCCT TCTGTTTGTG TCTACCTTGG ACGGAAGTCT CCATGCACTG AACAAGCAGA CAGGGGACTT GAAGTGGACA GTGAAGGATG ACCCCATCAT CCAGGGCCCA ATGTATGTCA CAGAAATGGC CTTTCTCTCT GACCCAGCTG ACGGCAGCCT GTATGTCTTG GGAACCCAGA AACAACAGGG GCTGATGAAA CTGCCATTCA CCATCCCAGA GCTGGTTCAT GCCTCTCCCT GTCGCAGCTC TGATGGTGTC TTCTACACAG GCCGGAAGCA GGATGCCTGG TTTGTAGTGG ACCCTGAGTC AGGGGAGACT CAGATGACAC TGACCACAGA AGGCCTCTCT ACTCCTCAAC TTTTCATTGG CCGAACACAG TACACAGTCT CCATGCATGA CCTACGGACC CCAGCCCTGC GGTGGAACAC AACCTATCGC CGCTACTCAG CACCCCTCCT GAATGGCTCA CCCGGGAAAT ACATGAGCCA CCTGACATCC TGTGGGATGG GCCTGCTTCT CACTGTGGAT CCAGGAAGTG GAATTGTGCT GTGGACACAG GACCTGGGAG TGCCTGTGAC AGGGATTTAC ACATGGCACC AGGATGGTCT GCACCAGCTG CCCCATCTCA CACTGGCTCG GGACACCCTA CATTTCCTTG TCCTCCGCTG GGGCCACATC CGTCTTCCTG CCTCCAGCTA CCAAGACACA GCCACCCAGT TCTCTTCCTT AGACACCCAG CTTTTGATGA CACTGTATGT GGGGAAAGAA GAAGCTGGGT TCTATGTCTC TAAAGCACTG GTCCACGCTG GGGTGGCCCT CGTGCCCCGT GGACTGACCC TGGCACCCAT GGACGGTCCC ACAACAGATG AGGTGACACT TCAAGTCTCG GGGGAGCGAG AGGGCTCACC CAGCACTGCT GTCAGATACC CCTCAGGCAG CGTGGCCCTC CCCAGCCAGT GGCTACTCAT TGGATACCAC GAACCTCCCC CCGTTCTACA CACAACCATG CTGCGGGTTC ATCCCATCCC AGGGAAAGTG TCTGCTGAGA CACGAGCCTC AGAGGACCTC CATGCTCCAC CTGTCTTTTT TGAGCTCCTA AACCTGAGGA GGGAGGATCC AGAACTACAT CCTGAAGAGA AAGCTTCAGA CTCTTATCCA GGGCTGGGAT CCCAAGACCT GCTAGCAGCT ACCTTCACTG CCATCCTCCT GGGAGCCTGG GTCCTCTACC TGATGAGGCA GCAGCAGCAG AGCCCTTCAG CACCTGCTGG CCCTCCTGAC CTCTCACAGG ATGCTCAGGG ACAGCTCTCA AGGGACATCT TACAGGACCA GAGGAGATTT CAAAGCCCCT CAGAGCCAGC CCAGCCACCT CATGACCCTG AAGGGCAGCC CACCGTGGTG GGGAAGATTT CCTTCAACCC CAAGGATGTG CTGGGCCGTG GGGCAGGAGG GACTTTTGTT TTCCGAGGAC AGTTTGAGGG GCGGGCAGTA GCTGTGGCCA GGCTCCTCCG AGAGTGCTTT GGCTTGGTAC GGCGAGAGGT GCAGCTGCTG CAAGAGTCTG ACCGGCACCC CAACGTGCTT CGCTACTTCT GCACTGAGCA TGGTCCCCAG TTCCACTACA TTGCCTTGGA GCTCTGCCAG GCCTCCTTGC AAGAGTACGT GGAGAGCCCA GACCTAGATC GCTGGGGCCT GGAGCCCACC ACAGTGCTGC AGCAGATGAT GTCCGGCCTG GCCCATCTAC ATTTCTTACA CATAGTACAC CGTGACCTAA AGCCAGCCAA CATTCTCATG GCTGGGCCTG ACAGCCAGGG CCAAGGCAGA GTGGTCATCT CTGACTTCGG CCTCTGCAAG AAGCTACCTG TTGGCCGTTG TAGCTTCAGT CTCCATTCTG GTATTCCCGG CACAGAAGGC TGGATGGCAC TAGAGCTCCT GCAGCTCCCC CCAGACAGCC CGACCAGCGC TGTGGATATC TTCTCCGCAG GCTGCGTATT TTATTACGTG CTTTCCGGTG GCAGCCACCC CTTTGGAGAG AGTCTCTACC GACAGGCCAA CATCCTCTCA GGGGATCCCT GTCTGGCTCA GCTGCAGGAA GAGACCCATG ACAAGGTTGT GGCATTGGAC TTAGTGAGAG CCATGCTGAG CTTGCTGCCC CAGGATCGCC CCTCGGCAGG ATGGGTACTG GCTCACCCCC TCTTTTGGAG CAGAGCTAAG GAGCTCCAGT TCTTCCAGGA TGTCAGTGAC TGGTTGGAGA AGGAACCAGA TCAGGGGCCT CTCGTGTCGG CGCTGGAGGC AGGAAGCTAC AAGGTGGTCC GGGAAGACTG GCACAAGCAC ATCTCAGCCC CTCTGCAGGC AGATCTGAAA AGGTTCCGCT CATACAAAGG GACATCAGTT CGAGACCTGC TCCGGGCCAT GAGGAACAAG AAGCACCACT ACAGGGAGCT CCCAGCTGAG GTGCGCCAGA CACTAGGCCA ACTTCCTGCT GGCTTCATTC AGTACTTCAC ACAGCGATTC CCCAGGCTGT TGCTGCACAC TCACCGTGCC ATGAGGACCT GCGCCTCTGA GAGCCTCTTC CTGCCCTACT ACCCACCAGC TCTGGAAGCC AGGAGGCCAG ATGCCACAAA GAGCGGAGGC GAGCAGAAGC TGATCTCTGA GGAGGATCTG TGACTCGAGC ATGCATCTAG AGGGCCCTAT TCTATAGTGT CACCTAAATG CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC