Ron Lab


 

pBABE retroviral plasmid tagged with farnysylated GFP

Plasmid was created in 1998 by Masahiko Kuroda.

The Puro resistance gene of pBABE puro was removed by Hind3-Cla1 digestion and replaced by the CDS of a EGFP that has a farnysylation signal in its C-term. The membrane association of EGFP is instrumental in detecting the protein in FACScan procedures that would otherwise lead to loss of GFP fluorescence.

Predicted sequence of the plasmid in EMBL format