Ron Lab


 

Plasmid Sequences

(instructions: how to request reagents from our collection)

Plasmids from our collection are now distributed through ADDGENE; we will no longer send out plasmids (except in special circumstances whereby Addgene is unable to do so).

AIRAP

AIRAP 1: Mouse AIRAP full length cDNA

Collection of plasmids used in Stanhill et al 2006 Mol Cell 23:875

ATF4

ATF4 1: Mouse ATF4 (CHOP11/cATF), wildtype, translational leader delete, co-expresses hCD2 as a transfection marker, mammalian expression vector

ATF4 2: Mouse ATF4 (CHOP11/cATF), bZIP domain only (i.e. lacking the N-terminal transactivation domain), co-expresses hCD2 as a transfection marker, mammalian expression vector

ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG ligated in frame with luciferase

ATF4 4-18: Collection of mouse ATF4 5'UTR reporter plasmids used in Lu et al 2004 J Cell Biol 167:27

ATF4 19: Mouse ATF4 (CHOP11/cATF), CDS (47 to C-term) fused to GST at its N-terminus

BiP (GRP78)

BiP 1: Mouse BiP cDNA (partial) PstI fragment in amp.resistant plasmid

BiP 2: C. elegans BiP (hsp-4) genomic reporter fused to GFP in a C. elegans expression plasmid

CHOP

CHOP 1: Mouse CHOP, wild-type, partial cDNA

CHOP 2: Mouse CHOP, wild-type, mammalian expression vector

CHOP 3: Mouse CHOP, delta leucine zipper mutant, mammalian expression vector

CHOP 4: Mouse CHOP, wild-type, 9E10-tagged retro-viral expression vector

CHOP 5: Mouse CHOP, delta leucine zipper mutant, retro-viral expression vector

CHOP 6: Mouse CHOP, wildtype, 9E10 (myc-epitope) tagged in pCDNA1 expression plasmid

CHOP 7: Mouse CHOP, wildtype, 9E10 (myc-epitope) tagged, fused to Gal4 DNA-BD in pCDNA1/amp expression plasmid

CHOP 8: Mouse CHOP, S78A & S81A double mutant, 9E10 (myc-epitope) tagged in pCDNA1/Amp expression plasmid (Note: there is some uncertainity surrounding the origin and identity of this plasmid)

CHOP 9: Mouse CHOP, S78A & S81A double mutant, 9E10 (myc-epitope) tagged, fused to Gal4 DNA-BD in pCDNA1/amp expression plasmid (Note: there is some uncertainity surrounding the origin and identity of this plasmid)

CHOP 10: CHOP::GFP reporter gene used in Novoa, et al., 2001, J Cell Biol; 153:1011

CReP

CReP 1: Mouse CReP (AA24-698), FLAG epitope tagged at N-terminus, CMV2-based mammalian expression plasmid

eIF2-alpha

eIF2α 1: h-eIF2alpha wildtype, mammalian expression vector

eIF2α 2: h-eIF2alpha S51A mutant, mammalian expression vector

eIF2α 3: h-eIF2alpha S51D mutant, mammalian expression vector

GADD34 (MyD116)

GADD34 1: Hamster GADD34 (MyD116) C-term fragment (A1 fragment), pBABE puro based mammalian expression plasmid

GADD34 2: Mouse GADD34 (MyD116), FLAG epitope tagged at N-terminus, CMV2-based mammalian expression plasmid

GADD34 3: Mouse GADD34 (MyD116) mutant lacking C-term PP1c-interacting domain, pBABE puro mammalian expression plasmid (a negative control)

GCN2

GCN2 1: Mouse GCN2, wild-type, FLAG-tagged at N-terminus, mammalian expression vector

GCN2 2: Mouse GCN2, K->M, FLAG-tagged at N-terminus, mammalian expression vector

GCN2 3: Mouse GCN2, kinase domain expressed as GST fusion protein, bacterial expression vector

GST

GST 1: GSTag: S. japonicum glutathione S-transferease tagged with a protein kinase A phosphorylation site

hCD2

hCD2 1: pCDNA3 plasmid, Neo resistance gene replaced by hCD2 (FACscan) marker

IRE1

IRE1 1: Human IRE1α, full-length coding cDNA in pcDNA1/amp (note: this plasmid is a gift of Randy Kaufman)

IRE1 2: Mouse IRE1β, full-length coding cDNA clone in Bluescript SK

IRE1 3: Mouse IRE1β, wild-type, mammalian expression vector

IRE1 4: Mouse IRE1β, wild-type, 9E10-tagged at C-term, mammalian expression vector

IRE1 5: Mouse IRE1β, K536A, 9E10-tagged at C-term, mammalian expression vector

IRE1 6: Mouse IRE1β, delta C-term, 9E10-tagged at C-term, mammalian expression vector

pBABE

pBABE 1: Retroviral vector tagged with farnysylated GFP

P58IPK-related

Collection of plasmids used in Oyadomari et al 2006 Cell 126:727

Protein disulfide isomerase (PDI1A, from Zito et al 2010)

PDI 1: Human PDI1A 18-508 wildtype pFLAG-CMV1 (FLAG M1 tagged)

PDI 2: Human PDI1A 18-508 C400S pFLAG-CMV1 (FLAG M1 tagged)

PDI 3: Human PDI1A 18-508 C56S; C400S pFLAG-CMV1 (FLAG M1 tagged)

PERK

PERK 1: Mouse PERK, wild-type, 9E10-tagged at C-terminus, mammalian expression vector

PERK 2: Mouse PERK, K618A, 9E10-tagged at C-terminus, mammalian expression vector

PERK 3: Mouse PERK, C-term deleted, 9E10-tagged at C-terminus, mammalian expression vector

PERK 4: Mouse PERK, wild-type, GST fusion to kinase domain, bacterial expression vector

PERK 5: Mouse PERK, K618R mutant, GST fusion to mutant kinase domain, bacterial expression vector

PERK 6: Mouse PERK, wildtype, 9E10-tagged in pBABE-puro

PERK 7: Mouse PERK, K618A, 9E10-tagged in pBABE-puro

PERK 8: Mouse PERK fused to Fv2E in pBABE-puro

PERK 9: Mouse PERK full-length cDNA in modified bluescript

SREC-like abu genes ( C. elegans)

SREC 1: abu-1::gfp C. elegans reporter gene

SREC 2: abu-1::abu-1::gfp C. elegans reporter gene

SV40 large T antigen for immortalizing cells

SV40 1: pBSSVD2005, a plasmid encoding SV-40 large T antigen

TLS & TLS-CHOP

TLS 1: Human TLS full-length cDNA in pBS KS+ cloning vector

TLS 2: Human TLS full-length cDNA in pCDNA1 mammalian expression vector

TLS 3: Human TLS full length cDNA in pRSET-C bacterial expression plasmid

TLS 4: Human TLS-CHOP fusion oncoprotein full-length cDNA in pBS KS+ cloning vector

TLS 5: Human TLS-CHOP fusion oncoprotein in pCDNA1 mammalian expression vector

XBP-1

XBP-1 1: Mouse, FLAG-tagged unspliced XBP-1 cDNA

XBP-1 2: Mouse, FLAG-tagged IRE1-spliced XBP-1 cDNA