Ron Lab


 

Cre PCR


10x buffer

100mM Tris-HCl pH 8.3

500mM KCl

15mM MgCl2


Reaction


10x buffer* 2.5ml
2.5mMdNTPmix 2.5 ml
Cre1S(10pmol/ ml) 1 ml
(GGG ATT GCT TAT AAC ACC CTG TTA CG)
Cre2AS(10pmol/ ml) 1 ml
(TAT TCG GAT CAT CAG CTA CAC CAG AG)
H2O 16 ml
Tail DNA 2 ml


Condition

94C 3min-(94C 1min- 60C 1min-72C 1min 30 cycle)-72 10min


PCR product:

213 bp


David Ron copied January 26, 2004
CRE PCR
(based on Masahiko's 1997 protocol)
Primers were ordered May 1997